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To do this, the inventors built a large plasmid (˜12 kb) exchanging the p15a-centered origin of replication through the primase origin. This plasmid targets the lacZ gene (p1322, SEQ ID NO: 25) in addition to has a chloramphenicol marker. as it was disregarded When the RBS toughness would want to get modified to copy a substantial plasmid, the inv

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